Keratin 77 抗体
Quick Overview for Keratin 77 抗体 (ABIN6941429)
抗原
See all Keratin 77 (KRT77) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
特异性
- This MAb recognizes the 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI , 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.
-
纯化方法
- Purified by Protein A/G
-
免疫原
- Human epidermal keratin
-
亚型
- IgG1 kappa
-
-
anti-Keratin 77 (KRT77) antibody
KRT77 适用: 人, 大鼠 IHC, StM 宿主: 小鼠 Monoclonal KRTL-1077 unconjugated
anti-Keratin 77 (KRT77) antibodyKRT77 适用: 人 IHC, StM 宿主: 兔 Monoclonal KRTL-1577R unconjugated Recombinant Antibody
anti-Keratin 77 (KRT77) (Internal Region) antibodyKRT77 适用: 人, 大鼠 IHC, ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-Keratin 77 (KRT77) (AA 1-578) antibodyKRT77 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Positive Control: Skin or squamous cell carcinoma (IHC).
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
-
限制
- 仅限研究用
-
-
-
浓度
- 200 μg/mL
-
缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-80 °C
-
储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
-
有效期
- 24 months
-
-
- Keratin 77 (KRT77)
-
别名
- KRT77
-
分子量
- 40-56.5kDa
-
基因ID
- 374454
-
UniProt
- Q7Z794
抗原
-
