ACTA1/ACTA2A/ACTG2 抗体
This anti-ACTA1/ACTA2A/ACTG2 antibody is a 小鼠 单克隆 antibody detecting ACTA1/ACTA2A/ACTG2 in IHC, StM 和 WB. Suitable for 人, 兔 和 大鼠.
Quick Overview for ACTA1/ACTA2A/ACTG2 抗体 (ABIN6941399)
抗原
ACTA1/ACTA2A/ACTG2
(ACTA1 (Skeletal), ACTA2 (Smooth), ACTG2 (Smooth))
适用
人, 兔, 大鼠
宿主
小鼠
克隆类型
单克隆
标记
This ACTA1/ACTA2A/ACTG2 antibody is un-conjugated
应用范围
Immunohistochemistry (IHC), Staining Methods (StM), Western Blotting (WB)
克隆位点
MSA-953
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特异性
- This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
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纯化方法
- Purified by Protein A/G
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免疫原
- Synthetic peptide common among human muscle actins
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亚型
- IgG1 kappa
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anti-ACTA1 (Skeletal), ACTA2 (Smooth), ACTG2 (Smooth) antibody
适用: 人, 兔, 大鼠, Cat, 小鸡, 犬, 小鼠 FACS, IF, IHC, StM 宿主: 小鼠 Monoclonal HHF35 unconjugated
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应用备注
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Positive Control: Muscle or sarcoma.
Known Application: Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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