Macrophage / Histiocytoma Marker 抗体
This anti-Macrophage / Histiocytoma Marker antibody is a 小鼠 单克隆 antibody detecting Macrophage / Histiocytoma Marker in IHC 和 StM. Suitable for 人.
Quick Overview for Macrophage / Histiocytoma Marker 抗体 (ABIN6941389)
抗原
Macrophage / Histiocytoma Marker
适用
人
宿主
小鼠
克隆类型
单克隆
标记
This Macrophage / Histiocytoma Marker antibody is un-conjugated
应用范围
Immunohistochemistry (IHC), Staining Methods (StM)
克隆位点
D11
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特异性
- In Western blotting, it detects an antigen of 125 kDa in human liver and 135 kDa in tumors of histiocytic origin. Comparative study of this MAb and a standard CD68 MAb showed that their antigens are different. Its antigen in all macrophage types studied is located on the plasma membrane and within cytoplasmic structures including lysosomes. This MAb shows a restricted reactivity to cells of the monocyte/macrophage system. It specifically reacts with blood monocytes and stains resident macrophages in a wide variety of human tissues. This MAb does not stain antigen-presenting cells, e.g., Langerhans cells. Reportedly, its reactivity is restricted to histiocytes and macrophages.
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无交叉反应
- 小鼠, 猪, 大鼠
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纯化方法
- Purified by Protein A/G
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免疫原
- Membrane preparation from human hepatocytes
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亚型
- IgG1 kappa
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anti-Macrophage / Histiocytoma Marker antibody
适用: 人 IHC, StM 宿主: 小鼠 Monoclonal LN-5 unconjugated
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应用备注
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Positive Control: Liver or Histiocytoma.
Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- 10 mM PBS with 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- Macrophage / Histiocytoma Marker
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别名
- Macrophage & Histiocytoma Marker
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分子量
- 125kDa -135kDa
抗原
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