FITC 抗体
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北京 101111
Quick Overview for FITC 抗体 (ABIN6941359)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- FITC (Fluorescein Isothiocyanate) Antibody
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特异性
- This MAb recognizes both the free and protein-conjugated (either soluble or cell bound) form of fluorescein or carboxy-fluorescein. Sensitivity of the FITC-anti-FITC system is reportedly similar to that of the biotin-avidin system. In biotin or enzyme-labeled form, this MAb is also suitable for the conversation of fluorescein-labeled detection into immuno-enzymatic assays.
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纯化方法
- 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.
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免疫原
- Fluorescein isothiocyanate (FITC) conjugated to KLH
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亚型
- IgG1, kappa
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应用备注
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Positive Control: Fluoresceinated proteins in solution or on tissues.
Known Application: Confocal Microscopy, Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), In situ Nucleic Acid Hybridization, Western Blot, Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) (Pretreatment depends upon the fluoreceinated primary antibody.)Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- FITC (Fluorescein Isothiocyanate (FITC))
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别名
- FITC (Fluorescein Isothiocyanate)
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物质类
- Chemical
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背景
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Fluorescein isothiocyanate, FITC
Fluoresceinated proteins in solution or on tissues.
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分子量
- 389.4Da (Molecular Formula: C21H11NO5S)
抗原
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