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FITC 抗体

The 小鼠 单克隆 anti-FITC antibody is suitable to detect FITC in samples from Various Species. It has been validated for WB, IHC, FACS 和 IF.
产品编号 ABIN6941359
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for FITC 抗体 (ABIN6941359)

抗原

FITC (Fluorescein Isothiocyanate (FITC))

适用

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Various Species

宿主

  • 43
  • 16
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小鼠

克隆类型

  • 54
  • 39
单克隆

标记

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This FITC antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

克隆位点

SPM395
  • 原理

    FITC (Fluorescein Isothiocyanate) Antibody

    特异性

    This MAb recognizes both the free and protein-conjugated (either soluble or cell bound) form of fluorescein or carboxy-fluorescein. Sensitivity of the FITC-anti-FITC system is reportedly similar to that of the biotin-avidin system. In biotin or enzyme-labeled form, this MAb is also suitable for the conversation of fluorescein-labeled detection into immuno-enzymatic assays.

    纯化方法

    200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

    免疫原

    Fluorescein isothiocyanate (FITC) conjugated to KLH

    亚型

    IgG1, kappa
  • 应用备注

    Positive Control: Fluoresceinated proteins in solution or on tissues.

    Known Application: Confocal Microscopy, Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), In situ Nucleic Acid Hybridization, Western Blot, Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) (Pretreatment depends upon the fluoreceinated primary antibody.)Optimal dilution for a specific application should be determined.

    限制

    仅限研究用
  • 浓度

    200 μg/mL

    缓冲液

    Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    有效期

    24 months
  • 抗原

    FITC (Fluorescein Isothiocyanate (FITC))

    别名

    FITC (Fluorescein Isothiocyanate)

    物质类

    Chemical

    背景

    Fluorescein isothiocyanate, FITC

    Fluoresceinated proteins in solution or on tissues.

    分子量

    389.4Da (Molecular Formula: C21H11NO5S)
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