KRT8, KRT18 抗体
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北京 101111
Quick Overview for KRT8, KRT18 抗体 (ABIN6941355)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells, there is very little keratin 18 in the normal epidermis so only Paget cells are stained.
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无交叉反应
- 小鸡, 驴, 雪貂, 小鼠, 兔, 大鼠, 非洲爪蟾
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纯化方法
- Purified by Protein A/G
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免疫原
- Cytoskeleton preparation from HeLa cells
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亚型
- IgG1 kappa
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anti-KRT8, KRT18 antibody
适用: 人 IHC, StM 宿主: 小鼠 Monoclonal KRT8-803-KRT18-835 unconjugated
anti-KRT8, KRT18 antibody适用: 人 IHC, StM 宿主: 小鼠 Monoclonal rKRT8-18-1346 unconjugated Recombinant Antibody
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应用备注
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Positive Control: MCF-7 or A431 cells. Skin, colon, lung or breast carcinoma.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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