Myeloid Cell Marker (Macrophage / Granulocyte Marker) 抗体
适用: 人
FACS, IF, IHC, StM, IHC (p), ISt
宿主: 小鼠
Monoclonal
BM-1
unconjugated
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- 抗原
- Myeloid Cell Marker (Macrophage / Granulocyte Marker)
- 适用
- 人
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- 非结合性
- 应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunostaining (ISt)
- 特异性
- Recognizes 183 kDa protein with DNA-binding characteristics, which is identified as a myeloid specific antigen. It reacts with myeloid precursor cells and granulocytes in bone marrow. Its antigen appears to be restricted to M2 and M3 acute myelogenous leukemia (AML) subtypes. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. This MAb reacts with early precursor and mature forms of human myeloid cells. It is useful in the identification of myelogenous leukemias, distinguishing granulocytic sarcomas from lymphoid malignancies and also in the study of differentiation and transformation of human myeloid cells. The biological function of this antigen is not clear, although it has been proposed that it may play a role in the differentiation of myeloid cells.
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Human peripheral blood mononuclear cells
- 克隆位点
- BM-1
- 亚型
- IgG1 kappa
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-
- 应用备注
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Positive Control: HL60 cells. Bone marrow, lymph node or tonsil.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) (No special pretreatment is required for staining of formalin/paraffin tissues.)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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- 抗原
- Myeloid Cell Marker (Macrophage / Granulocyte Marker)
- 分子量
- 183kDa
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