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- 抗原
- Proliferation Marker
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Staining Methods (StM)
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Pokeweed-stimulated lymphocytes
- 克隆位点
- JC1
- 亚型
- IgG2a kappa
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anti-Proliferation Marker antibody
适用: 人 IHC, WB, ELISA, IHC (fro), IHC (p), IP 宿主: 小鼠 Monoclonal IPO-38 unconjugated
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- 应用备注
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Positive Control: HeLa cells (IF). Human lymph node (IHC).
Known Application: Western Blot (0.5-2 μg/mL), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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- 抗原
- Proliferation Marker
- 背景
- JC1 detects a nuclear antigen, distinct from Ki67, present in proliferating cells. It is useful as a marker of proliferation in cases such as squamous cell carcinoma where the Ki67 index cannot be determined.
- 分子量
- 212kDa and 123kDa
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