-
- 抗原
- Myofibroblast Marker
- 适用
- 人
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- 非结合性
- 应用范围
- Immunohistochemistry (IHC), Staining Methods (StM), Western Blotting (WB)
- 特异性
- Myofibroblasts are a unique group of smooth muscle-like fibroblasts that play an important role in oncogenesis, inflammation, repair, wound contraction and fibrosis. Like smooth muscle (SM) cells, myofibroblasts contain microfilament bundles and express -SM Actin, the Actin isoform that is present in myoepithelial cells and SM cells and especially abundant in vascular SM cells. Myofibroblasts secrete inflammatory and anti-inflammatory cytokines, chemokines, growth factors and lipid and gaseous inflammatory mediators, as well as extracellular matrix proteins and proteases in most organs and tissues. Besides being temporarily present following tissue injuries and fibrocontractive diseases, myofibroblasts are also present under normal conditions in regions such as the skin, pulmonary septa and periodontal ligaments. Stem cell factor and platelet-derived growth factor (PDGF) are two secreted proteins responsible for differentiating myofibroblasts from embryological stem cells. PR 2D3 reacts with a cell membrane component of cells in the pericrypt sheath, with smooth muscle cells and myofibroblasts. This antibody is considered to be the gold standard for the identification of myofibroblasts.
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Crude homogenate of normal human colorectal mucosa
- 克隆位点
- PR 2D3
- 亚型
- IgG1 kappa
-
-
- 应用备注
-
Positive Control: Myofibroblasts.
Known Application: Western Blot (0.5-1.0 μg/mL) Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
-
- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
-
- 抗原
- Myofibroblast Marker
- 分子量
- 28kDa
-
You are here:
