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CD47 抗体 (AA 18-135)

This anti-CD47 antibody is a 小鼠 单克隆 antibody detecting CD47 in FACS, IHC, IF, ELISA, StM 和 Coat. Suitable for 人.
产品编号 ABIN6941215
发货至: 中国

Quick Overview for CD47 抗体 (AA 18-135) (ABIN6941215)

抗原

See all CD47 抗体
CD47

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This CD47 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Staining Methods (StM), Coating (Coat)

克隆位点

CD47-3019
  • 抗原表位

    • 24
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    • 8
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    AA 18-135

    特异性

    This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50 kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.

    纯化方法

    Purified by Protein A/G

    免疫原

    A recombinant fragment (around aa 18-135) of human CD47 protein (exact sequence is proprietary)

    亚型

    IgG1 kappa
  • 应用备注

    Positive Control: MCF-7, HUVEC or OVCA3 cells (IF/FACS). Human placenta, brain or ovarian tumors (IHC).

    Known Application: ELISA (For coating, order antibody without BSA),Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    限制

    仅限研究用
  • 浓度

    200 μg/mL

    缓冲液

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    有效期

    24 months
  • 抗原

    CD47

    别名

    CD47

    分子量

    ~50kDa

    基因ID

    961

    UniProt

    Q08722
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