CD47 抗体
Quick Overview for CD47 抗体 (ABIN6941211)
抗原
See all CD47 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
特异性
- This antibody reacts with Ig domain of CD47 protein. It has been shown to inhibit polymorphonuclear neutrophil (PMN) transmigration across cell monolayers and matrix. CD47, originally named integrin-associated protein (IAP), is a 50 kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.
-
纯化方法
- Purified by Protein A/G
-
免疫原
- Intact CD47 Purified from placenta
-
亚型
- IgG1 kappa
-
-
-
-
应用备注
-
Positive Control: MCF-7, HUVEC, OVCA3 cells, Placenta, Brain and Ovarian Tumors.
Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
-
限制
- 仅限研究用
-
-
-
浓度
- 200 μg/mL
-
缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-80 °C
-
储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
-
有效期
- 24 months
-
-
- CD47
-
别名
- CD47
-
分子量
- ~50kDa
-
基因ID
- 961
-
UniProt
- Q08722
抗原
-