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CD5 抗体 (AA 269-366)

Name: CD5 抗体 (AA 269-366)
Brand: antibodies-online
SKU: ABIN6941056
Availability: OutOfStock

This anti-CD5 antibody is a 小鼠单克隆 antibody detecting CD5 in FACS, WB, IHC, IF 和 StM. Suitable for 人.

Flow Cytometric Analysis of human Jurkat cells using CD5-Monospecific Mouse Monoclonal Antibody (CD5/2416) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red). (CD5 抗体 (AA 269-366))

FACS

Western Blot Analysis of human Jurkat cell lysate using CD5-Monospecific Mouse Monoclonal Antibody (CD5/2416). (CD5 抗体 (AA 269-366))

Analysis of Protein Array containing more than 19,000 full-length human proteins using Mouse CD5-Monospecific Monoclonal Antibody (CD5/2416) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. (CD5 抗体 (AA 269-366))

PAr

Flow Cytometric Analysis of Ramos cells using CD5-Monospecific Mouse Monoclonal Antibody (CD5/2416) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red). (CD5 抗体 (AA 269-366))

FACS

Formalin-fixed, paraffin-embedded human Tonsil stained with CD5-Monospecific Mouse Monoclonal Antibody (CD5/2416). (CD5 抗体 (AA 269-366))

IHC

Immunofluorescent staining of PFA-fixed Ramos cells using CD5-Monospecific Mouse Monoclonal Antibody (CD5/2416) followed by goat anti-Mouse IgG conjugated to CF488 (green). (CD5 抗体 (AA 269-366))

SDS-PAGE Analysis Purified CD5-Monospecific Mouse Monoclonal Antibody (CD5/2416). (CD5 抗体 (AA 269-366))

SDS

7 images

产品编号 ABIN6941056

发货至: 中国

Datasheet as PDF

Quick Overview for CD5 抗体 (AA 269-366) (ABIN6941056)

抗原

See all CD5 抗体

CD5

适用

人

宿主

小鼠

克隆类型

单克隆

标记

This CD5 antibody is un-conjugated

应用范围

Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Staining Methods (StM)

克隆位点

CD5-2416

Discover our top product CD5 Primary Antibody

抗原表位
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AA 269-366

特异性

Recognizes a 67 kDa transmembrane protein, which is identified as CD5. The CD5 antigen is found on 95 % of thymocytes and 72 % of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10 %) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms. Anti-CD5 detection is diagnostic in CLL/SLL within a panel of other B-cell markers, especially one that includes anti-CD23. Anti-CD5 is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, anti-CD5 can be used in distinguishing thymic carcinoma (+) from thymoma (-). Anti-CD5 does not react with granulocytes or monocytes.

纯化方法

Purified by Protein A/G

免疫原

Recombinant fragment of human CD5 protein (around aa 269-366) (exact sequence is proprietary)

亚型

IgG2b kappa
anti-CD5 (CD5) (AA 105-210) antibody
CD5 适用: 人 WB 宿主: 山羊 Polyclonal unconjugated

anti-CD5 (CD5) (AA 269-366) antibody
CD5 适用: 人 FACS, IHC, IF, StM 宿主: 小鼠 Monoclonal CD5-2418 unconjugated

anti-CD5 (CD5) antibody
CD5 适用: 人 FACS, WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal L17F12 unconjugated

anti-CD5 (CD5) (AA 403-495) antibody
CD5 适用: 人, 小鼠 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 6C3B2 unconjugated

anti-CD5 (CD5) (AA 27-233) antibody
CD5 适用: 人 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 6A11 unconjugated

anti-CD5 (CD5) (AA 403-495) antibody
CD5 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2F7 unconjugated

anti-CD5 (CD5) antibody
CD5 适用: 人 FACS, WB, IP, ELISA, IHC (p) 宿主: 小鼠 Monoclonal MEM-32 unconjugated

anti-CD5 (CD5) antibody
CD5 适用: 人 FACS, WB, IP, ELISA, IHC (fro) 宿主: 小鼠 Monoclonal CRIS1 unconjugated

anti-CD5 (CD5) antibody (PE)
CD5 适用: 人 FACS 宿主: 小鼠 Monoclonal L17F12 PE

anti-CD5 (CD5) (AA 25-372) antibody
CD5 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 3D3G6 unconjugated

应用备注

Positive Control: 293T, Ramos or MOLT-4 cells. Tonsil or Lymph Node.
Known Application: Flow Cytometry (1-2 μg/million cells), Western Blot (1-2 μg/mL), Immunofluorescence (1-2 μg/million cells),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

限制

仅限研究用
浓度

200 μg/mL

缓冲液

10 mM PBS with 0.05 % BSA & 0.05 % azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-80 °C

储存方法

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

有效期

24 months
抗原

CD5

别名

CD5

分子量

67kDa

基因ID

921

UniProt

P06127