MAML2 抗体
Quick Overview for MAML2 抗体 (ABIN6940961)
抗原
See all MAML2 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Purified by Protein A/G
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免疫原
- Recombinant human MAML2 fragment
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亚型
- IgG2a kappa
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anti-Mastermind-Like 2 (MAML2) (AA 347-506) antibody
MAML2 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Mastermind-Like 2 (MAML2) (AA 796-894) antibodyMAML2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4A1 unconjugated
anti-Mastermind-Like 2 (MAML2) (AA 780-814) antibodyMAML2 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB52651 unconjugated
anti-Mastermind-Like 2 (MAML2) (AA 750-850) antibodyMAML2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Mastermind-Like 2 (MAML2) (AA 324-373) antibodyMAML2 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Mastermind-Like 2 (MAML2) (AA 169-218) antibodyMAML2 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Positive Control: MCF-7 cells. Pancreas or Placenta. Bladder or Colon Carcinoma.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- MAML2 (Mastermind-Like 2 (MAML2))
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别名
- MAML2
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背景
- MAML2 (mastermind-like protein 2), also known as MAM2, MAM3 or MLLMAML2, is a nuclear speckle protein that acts as a transcriptional co-activator for Notch receptors. The Notch signaling pathway influences cell fate by regulating the ability of precursor cells to properly respond to developmental signals. MAML2 is a member of the mastermind-like family of proteins that are human homologs of the Drosophila melanogaster mastermind protein. Through its N-terminal region, MAML2 interacts with the ankyrin repeats of the Notch proteins Notch 1, Notch 2, Notch 3 and Notch 4. This interaction leads to formation of a DNA-binding complex with the Notch proteins and RBP-J a complex that can then induce HES1 gene expression. While the N-terminal domain of MAML2 is essential for proper Notch binding, the C-terminal domain of MAML2 is essential for transcriptional activation. A chromosomal aberration involving the gene encoding MAML2 is implicated in mucoepidermoid carcinomas, clear cell hidradenomas and benign Warthin tumors.
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分子量
- 125kDa
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基因ID
- 84441
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UniProt
- Q8IZL2
抗原
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