PAX8 抗体 (AA 60-261)
Quick Overview for PAX8 抗体 (AA 60-261) (ABIN6940924)
抗原
See all PAX8 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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                                            抗原表位
- AA 60-261
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                                            特异性
- Recognizes a protein of 62 kDa, identified as PAX8. It is a member of the paired box (PAX) family of transcription factors. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas, and atypical thyroid adenomas. PAX-8 is expressed in the thyroid (and associated carcinomas), non-ciliated mucosal cells of the fallopian tubes, and simple ovarian inclusion cysts, but not normal ovarian surface epithelial cells. PAX-8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas. PAX-8 expression is reported in renal tubules as well as renal cell carcinoma, nephroblastoma, and seminoma. PAX-8 antibody may be used as an additional immunohistochemical marker for renal epithelial tumors.
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                                            纯化方法
- Purified by Protein A/G
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                                            免疫原
- Recombinant fragment (around aa 60-261) of human PAX8 protein (exact sequence is proprietary)
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                                            亚型
- IgG2b kappa
 
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                                            应用备注
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                        Positive Control: Raji cells. Renal Cell Carcinoma (RCC). Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined. 
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                                            限制
- 仅限研究用
 
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                                            浓度
- 200 μg/mL
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                                            缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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                                            储存液
- Sodium azide
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                                            注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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                                            储存条件
- 4 °C,-80 °C
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                                            储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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                                            有效期
- 24 months
 
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    - PAX8 (Paired Box 8 (PAX8))
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                                            别名
- PAX8
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                                            分子量
- 62kDa
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                                            基因ID
- 7849
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                                            UniProt
- Q06710
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                                            途径
- Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Stem Cell Maintenance, Tube Formation
 抗原
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