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CA9 抗体

This anti-CA9 antibody is a Mouse Monoclonal antibody detecting CA9 in WB, IHC, IF, FACS, StM. Suitable for Human, Horse.
产品编号 ABIN6940921
发货至: 中国

Quick Overview for CA9 抗体 (ABIN6940921)

抗原

See all CA9 抗体
CA9 (Carbonic Anhydrase IX (CA9))

适用

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人, 马

宿主

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小鼠

克隆类型

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单克隆

标记

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This CA9 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

克隆位点

PN-15
  • 特异性

    Recognizes a glycoprotein of ~200 kDa, identified as carbonic anhydrase IX (CAIX/gp200). Its epitope resides in the carbohydrate domain of gp200. It shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG, and AB blood group antigens. In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, gp200 is expressed by 93 % of primary and 84 % of metastatic renal cell carcinomas. This MAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.

    纯化方法

    Purified by Protein A/G

    免疫原

    Microsomal fraction of human renal cortical tissue homogenate

    亚型

    IgG2b kappa
  • 应用备注

    Positive Control: Normal kidney or renal cell carcinoma.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    限制

    仅限研究用
  • 浓度

    200 μg/mL

    缓冲液

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    有效期

    24 months
  • 抗原

    CA9 (Carbonic Anhydrase IX (CA9))

    别名

    CA9

    分子量

    55kDa

    基因ID

    768

    UniProt

    Q16790
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