Villin 1 抗体 (AA 179-311)
Quick Overview for Villin 1 抗体 (AA 179-311) (ABIN6940871)
抗原
See all Villin 1 (VIL1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 179-311
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特异性
- Recognizes a protein of 95 kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.
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纯化方法
- Purified by Protein A/G
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免疫原
- Recombinant human Villin fragment (around aa179-311) (exact sequence is proprietary)
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亚型
- IgG1 kappa
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anti-Villin 1 (VIL1) (AA 1-320) antibody
VIL1 适用: 人 IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Villin 1 (VIL1) (N-Term) antibodyVIL1 适用: 人 WB, IF, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Villin 1 (VIL1) (AA 1-209) antibodyVIL1 适用: 人 IHC, WB, FACS, ELISA, ICC 宿主: 小鼠 Monoclonal 3E5G11 unconjugated
anti-Villin 1 (VIL1) (AA 1-209) antibodyVIL1 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 5E3B2 unconjugated
anti-Villin 1 (VIL1) (AA 180-207), (N-Term) antibodyVIL1 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB17824 unconjugated
anti-Villin 1 (VIL1) (AA 1-421) antibodyVIL1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Villin 1 (VIL1) (AA 1-421) antibodyVIL1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2F10 unconjugated
anti-Villin 1 (VIL1) (AA 179-311) antibodyVIL1 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal rVIL1-1325 unconjugated Recombinant Antibody
anti-Villin 1 (VIL1) (AA 600-700) antibodyVIL1 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-459R unconjugated Recombinant Antibody
anti-Villin 1 (VIL1) (AA 770-799), (C-Term) antibodyVIL1 适用: 人, 小鼠, 大鼠 IHC, WB, IF, FACS 宿主: 兔 Polyclonal unconjugated
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应用备注
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Positive Control: A549, HepG2 and HCT116 cells. Colon or Rectum.
Known Application: Western Blot (1-2 μg/mL for 60 minutes at RT), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- Villin 1 (VIL1)
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别名
- VIL1
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分子量
- 93kDa
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基因ID
- 7429
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UniProt
- P09327
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途径
- EGFR Signaling Pathway, Regulation of Actin Filament Polymerization
抗原
-
