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VCAM1 抗体

The 小鼠 单克隆 anti-VCAM1 antibody (Clone 1-4C3) (ABIN6940861) specifically detects VCAM1 in FACS, IHC 和 IF. The antibody is reactive with 人 samples.
产品编号 ABIN6940861
发货至: 中国
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Quick Overview for VCAM1 抗体 (ABIN6940861)

抗原

See all VCAM1 抗体
VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This VCAM1 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

克隆位点

1-4C3
  • 原理

    CD106 / VCAM1 (Activated Endothelial Cell Marker) Antibody

    特异性

    Recognizes a protein of 110 kDa, identified as CD106 (also known as vascular cell adhesion molecule-1 (VCAM-1) and INCAM-100). CD106 is a member of the Ig superfamily of adhesion molecules and is expressed at high levels on cytokine stimulated vascular endothelial cells, and at minimal levels on un-stimulated endothelial cells. It is also present on follicular and inter-follicular dendritic cells of lymph nodes, myoblasts, and some macrophages. CD106 serves as a ligand for leukocyte integrin (VLA-4 or CD49d/CD29) and mediates cell adhesion of leukocytes to activated endothelium. It plays a role in various immunological and inflammatory responses.

    纯化方法

    200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

    免疫原

    Stimulated human umbilical vein endothelial cells (HUVEC)

    亚型

    IgG1, kappa
  • 应用备注

    Positive Control: Human placenta or tonsil.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    限制

    仅限研究用
  • 浓度

    200 μg/mL

    缓冲液

    Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    有效期

    24 months
  • 抗原

    VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

    别名

    VCAM1

    背景

    CD106, INCAM-100, L1CAM, Vascular Cell Adhesion Molecule 1 (VCAM1), Vascular cell adhesion protein 1

    Human placenta or tonsil.

    分子量

    110kDa

    基因ID

    7412, 109225

    UniProt

    P19320

    途径

    Carbohydrate Homeostasis
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