TYMS 抗体 (AA 60-174)
Quick Overview for TYMS 抗体 (AA 60-174) (ABIN6940822)
抗原
See all TYMS 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 60-174
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特异性
- It recognizes a protein of 36 kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). It converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein has been reported to associate with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.
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纯化方法
- Purified by Protein A/G
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免疫原
- Recombinant human thymidylate synthase protein fragment (around aa 60-174) (exact sequence is proprietary)
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亚型
- IgG2c kappa
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anti-Thymidylate Synthetase (TYMS) (AA 36-313) antibody
TYMS 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Thymidylate Synthetase (TYMS) (AA 111-210) antibodyTYMS 适用: 人 WB, IF, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3A1 unconjugated
anti-Thymidylate Synthetase (TYMS) antibodyTYMS 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 1D6 unconjugated
anti-Thymidylate Synthetase (TYMS) antibodyTYMS 适用: 人 WB, IF, IHC (p), IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Thymidylate Synthetase (TYMS) antibodyTYMS 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 5H8 unconjugated
anti-Thymidylate Synthetase (TYMS) (AA 111-210) antibodyTYMS 适用: 人 WB, ELISA, IHC (p), IP 宿主: 小鼠 Monoclonal 2B2 unconjugated
anti-Thymidylate Synthetase (TYMS) (AA 265-294), (C-Term) antibodyTYMS 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB20041 unconjugated
anti-Thymidylate Synthetase (TYMS) (AA 2-54) antibodyTYMS 适用: 人 WB, IF, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-Thymidylate Synthetase (TYMS) (AA 60-174) antibodyTYMS 适用: 人 IF, FACS, ELISA, RIA, Coat 宿主: 小鼠 Monoclonal rTYMS-1884 unconjugated Recombinant Antibody
anti-Thymidylate Synthetase (TYMS) (AA 1-313), (N-Term) antibodyTYMS 适用: 人 WB, IF, EIA 宿主: 小鼠 Monoclonal AT1S5 unconjugated
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应用备注
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Positive Control: MOLT4, Ramos, 5-FU-resistant colon carcinoma cell lines (NCI H630R10, NCI H630R1), 5-FU-resistant breast cancer cell lines, MCF-Ad5 and MCF-Ad10. Testicular carcinomas.
Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. ,Non-hazardous. No MSDS required.
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有效期
- 24 months
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- TYMS (Thymidylate Synthetase (TYMS))
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别名
- TYMS
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分子量
- 36kDa
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基因ID
- 7298
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UniProt
- P04818
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途径
- Mitotic G1-G1/S Phases
抗原
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