Transferrin Receptor 抗体 (AA 94-212)
Quick Overview for Transferrin Receptor 抗体 (AA 94-212) (ABIN6940699)
抗原
See all Transferrin Receptor (TFRC) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
抗原表位
- AA 94-212
-
特异性
- It recognizes a ~90-95 kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190 kDa. This MAb is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.
-
纯化方法
- Purified by Protein A/G
-
免疫原
- Recombinant extracellular fragment (around aa 94-212) of human TFRC protein (exact sequence is proprietary)
-
亚型
- IgG2b kappa
-
-
-
-
应用备注
-
Positive Control: Jurkat, HeLa, MCF-7 or K562 cells. Human placenta or Bone Marrow.
Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes) Western Blot (0.5-1.0 μg/mL)Optimal dilution for a specific application should be determined.
-
限制
- 仅限研究用
-
-
-
浓度
- 200 μg/mL
-
缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-80 °C
-
储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
-
有效期
- 24 months
-
-
- Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))
-
别名
- TFRC
-
分子量
- 85-95kDa (monomer), 190kDa (dimer)
-
基因ID
- 7037
-
UniProt
- P02786
-
途径
- Transition Metal Ion Homeostasis
抗原
-