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Transferrin Receptor 抗体 (AA 94-212)

This anti-Transferrin Receptor antibody (ABIN6940699) is a Mouse Monoclonal antibody detecting Transferrin Receptor in WB, IHC, ELISA, Coat, StM. Suitable for Human.
产品编号 ABIN6940699
发货至: 中国

Quick Overview for Transferrin Receptor 抗体 (AA 94-212) (ABIN6940699)

抗原

See all Transferrin Receptor (TFRC) 抗体
Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

适用

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小鼠

克隆类型

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单克隆

标记

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This Transferrin Receptor antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Coating (Coat), Staining Methods (StM)

克隆位点

TFRC-1817
  • 抗原表位

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    AA 94-212

    特异性

    It recognizes a ~90-95 kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190 kDa. This MAb is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.

    纯化方法

    Purified by Protein A/G

    免疫原

    Recombinant extracellular fragment (around aa 94-212) of human TFRC protein (exact sequence is proprietary)

    亚型

    IgG2b kappa
  • 应用备注

    Positive Control: Jurkat, HeLa, MCF-7 or K562 cells. Human placenta or Bone Marrow.

    Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes) Western Blot (0.5-1.0 μg/mL)Optimal dilution for a specific application should be determined.

    限制

    仅限研究用
  • 浓度

    200 μg/mL

    缓冲液

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    有效期

    24 months
  • 抗原

    Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

    别名

    TFRC

    分子量

    85-95kDa (monomer), 190kDa (dimer)

    基因ID

    7037

    UniProt

    P02786

    途径

    Transition Metal Ion Homeostasis
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