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CD43 抗体

SPN 适用: 人 FACS, IHC, IF, StM 宿主: 小鼠 Monoclonal Bra7G unconjugated
产品编号 ABIN6940638
发货至: 中国
  • 抗原 See all CD43 (SPN) 抗体
    CD43 (SPN) (Sialophorin (SPN))
    适用
    • 153
    • 9
    • 9
    宿主
    • 96
    • 67
    • 3
    小鼠
    克隆类型
    • 85
    • 80
    • 1
    单克隆
    标记
    • 61
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    This CD43 antibody is un-conjugated
    应用范围
    • 103
    • 75
    • 62
    • 61
    • 39
    • 17
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    • 5
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    Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Staining Methods (StM)
    特异性
    It recognizes a cell surface glycoprotein of 95/115/135 kDa (depending upon the extent of glycosylation), identified as CD43 (Workshop V). Epitope of MAb Bra7G is clearly different from that of MAb DF-T1, called b as opposed to a for DF-T1. 70-90 % of T-cell lymphomas and from 22-37 % of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
    免疫原
    Immature pluripotent human leukemia K562 cells
    克隆位点
    Bra7G
    亚型
    IgM kappa
    Top Product
    Discover our top product SPN Primary Antibody
  • 应用备注

    Positive Control: K562 cells, Paracortex in a tonsil or a reactive lymph node.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT),(For staining of formalin-fixed tissues, digest sections with trypsin at 1 mg/mL PBS, 15 min at room temp),Optimal dilution for a specific application should be determined.

    限制
    仅限研究用
  • 浓度
    200 μg/mL
    缓冲液
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-80 °C
    储存方法
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    有效期
    24 months
  • 抗原
    CD43 (SPN) (Sialophorin (SPN))
    别名
    SPN (SPN 产品)
    别名
    CD43 antibody, GALGP antibody, GPL115 antibody, LSN antibody, A630014B01Rik antibody, Cd43 antibody, Galgp antibody, Ly-48 antibody, Ly48 antibody, Lsn antibody, Lsn1 antibody, gpL115 antibody, sialophorin antibody, SPN antibody, Spn antibody
    分子量
    95, 115, or 135kDa
    基因ID
    6693
    UniProt
    P16150
    途径
    Regulation of Leukocyte Mediated Immunity
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