CD43 抗体
Quick Overview for CD43 抗体 (ABIN6940638)
抗原
See all CD43 (SPN) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- It recognizes a cell surface glycoprotein of 95/115/135 kDa (depending upon the extent of glycosylation), identified as CD43 (Workshop V). Epitope of MAb Bra7G is clearly different from that of MAb DF-T1, called b as opposed to a for DF-T1. 70-90 % of T-cell lymphomas and from 22-37 % of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
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免疫原
- Immature pluripotent human leukemia K562 cells
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亚型
- IgM kappa
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应用备注
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Positive Control: K562 cells, Paracortex in a tonsil or a reactive lymph node.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT),(For staining of formalin-fixed tissues, digest sections with trypsin at 1 mg/mL PBS, 15 min at room temp),Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- CD43 (SPN) (Sialophorin (SPN))
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别名
- SPN
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分子量
- 95, 115, or 135kDa
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基因ID
- 6693
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UniProt
- P16150
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途径
- Regulation of Leukocyte Mediated Immunity
抗原
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