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CD43 抗体

This anti-CD43 antibody is a 小鼠 单克隆 antibody detecting CD43 in FACS, IHC, IF 和 StM. Suitable for 人.
产品编号 ABIN6940638
发货至: 中国

Quick Overview for CD43 抗体 (ABIN6940638)

抗原

See all CD43 (SPN) 抗体
CD43 (SPN) (Sialophorin (SPN))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This CD43 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Staining Methods (StM)

克隆位点

Bra7G
  • 特异性

    It recognizes a cell surface glycoprotein of 95/115/135 kDa (depending upon the extent of glycosylation), identified as CD43 (Workshop V). Epitope of MAb Bra7G is clearly different from that of MAb DF-T1, called b as opposed to a for DF-T1. 70-90 % of T-cell lymphomas and from 22-37 % of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.

    免疫原

    Immature pluripotent human leukemia K562 cells

    亚型

    IgM kappa
  • 应用备注

    Positive Control: K562 cells, Paracortex in a tonsil or a reactive lymph node.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT),(For staining of formalin-fixed tissues, digest sections with trypsin at 1 mg/mL PBS, 15 min at room temp),Optimal dilution for a specific application should be determined.

    限制

    仅限研究用
  • 浓度

    200 μg/mL

    缓冲液

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    有效期

    24 months
  • 抗原

    CD43 (SPN) (Sialophorin (SPN))

    别名

    SPN

    分子量

    95, 115, or 135kDa

    基因ID

    6693

    UniProt

    P16150

    途径

    Regulation of Leukocyte Mediated Immunity
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