Spastin 抗体
Quick Overview for Spastin 抗体 (ABIN6940629)
抗原
See all Spastin (SPAST) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
纯化方法
- Purified by Protein A/G
-
免疫原
- Recombinant full-length human Spastin protein.
-
亚型
- IgG2a kappa
-
-
anti-Spastin (SPAST) (AA 200-304) antibody
SPAST 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 2F5 unconjugated
anti-Spastin (SPAST) (AA 551-616) antibodySPAST 适用: 小鼠, 大鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Spastin (SPAST) antibodySPAST 适用: 小鼠, 大鼠, 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Spastin (SPAST) (AA 200-304) antibodySPAST 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3A12 unconjugated
-
-
应用备注
-
Positive Control: HeLa cells. Rat brain extract.
Known Application: ELISA (For coating, order antibody without BSA), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
-
限制
- 仅限研究用
-
-
-
浓度
- 200 μg/mL
-
缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-80 °C
-
储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
-
有效期
- 24 months
-
-
- Spastin (SPAST)
-
别名
- SPAST
-
背景
- The AAA protein family members share an ATPase domain and have roles in various cellular processes including intracellular motility, membrane trafficking, proteolysis, protein folding and organelle biogenesis. Spastin, a member of the AAA protein family, is a 616 amino acid protein and is involved in the function or assembly of nuclear protein complexes. The Spastin protein is expressed ubiquitously and localizes to the nucleus and the cytoplasm, where it may also be involved in microtubule dynamics. Mutations in the Spastin gene (SPAST, SPG4) cause the most common form of spastic paraplegia 4, an autosomal dominant form of hereditary spastic paraplegia (HSP). HSPs comprise a group of inherited neurological disorders characterized by spastic lower extremity weakness due to a length-dependent, retrograde axonopathy of corticospinal motor neurons. SPAST-specific mutations account for approximately 40 % of all autosomal dominant HSPs.
-
分子量
- 52kDa
-
基因ID
- 6683
-
途径
- Microtubule Dynamics, M Phase, Regulation of Cell Size
抗原
-
