Sialoadhesin/CD169 抗体 (N-Term)
Quick Overview for Sialoadhesin/CD169 抗体 (N-Term) (ABIN6940605)
抗原
See all Sialoadhesin/CD169 (SIGLEC1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- N-Term
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纯化方法
- Purified by Protein A/G
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免疫原
- Fc fusion protein containing N-terminal 4 domains of human sialoadhesin.
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亚型
- IgG1 kappa
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anti-Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1) (AA 851-950) antibody
SIGLEC1 适用: 人, 大鼠 FACS, ELISA, IHC (fro), ICC, IF (cc), IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1) (AA 20-197) antibodySIGLEC1 适用: 人 FACS, ELISA, ICC 宿主: 小鼠 Monoclonal 2A4D1 unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1) (AA 572-697) antibodySIGLEC1 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1) antibody (APC)SIGLEC1 适用: 人 FACS 宿主: 小鼠 Monoclonal 7-239 APC
anti-Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1) antibodySIGLEC1 适用: 大鼠 FACS, IP, IHC (fro), IF 宿主: 小鼠 Monoclonal ED3 unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1) antibodySIGLEC1 适用: 人 FACS, WB, IP, IHC (fro) 宿主: 小鼠 Monoclonal 7-239 unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1) antibody (PE)SIGLEC1 适用: 人 FACS 宿主: 小鼠 Monoclonal 7-239 PE
anti-Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1) (AA 851-950) antibody (AbBy Fluor® 647)SIGLEC1 适用: 人, 大鼠 FACS, IF (cc), IF (p) 宿主: 兔 Polyclonal AbBy Fluor® 647
anti-Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1) (Internal Region) antibodySIGLEC1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1) antibodySIGLEC1 适用: 小鼠 WB, IHC 宿主: 大鼠 Monoclonal 3D6-112 unconjugated Recombinant Antibody
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应用备注
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Positive Control: Human spleen, human peripheral blood monocytes or MCF7 cell.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- Sialoadhesin/CD169 (SIGLEC1) (Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1))
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别名
- SIGLEC1
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背景
- Two families of mammalian lectin-like adhesion molecules, the selectins and the sialoadhesins, bind glycoconjugate ligands in a sialic acid-dependent manner. The sialic acid-binding immunoglobulin superfamily lectins, designated siglecs or sialoadhesins, are immunoglobulin superfamily members that recognize sialylated ligands. The common sialic acids of mammalian cells are N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc). The human Siglec-1 gene maps to chromosome 20p13 and encodes a 1,709 amino acid protein, also known as CD169. Alternative splicing of the Siglec-1 gene produces a variant, encoding a type I transmembrane protein isoform that is soluble rather than membrane-bound. Studies have shown human Siglec-1 has greater affinity for Neu5Ac over Neu5Gc. Siglec-1 is a sialic acid-binding receptor that is expressed in hemopoietic cells. It mediates local cell-cell interactions in lymphoid tissues and can be detected at contact points of macrophages with other macrophages, sinus-lining cells and reticulum cells.
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分子量
- 185kDa
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基因ID
- 6614
抗原
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