s100a4 抗体 (AA 1-200)
Quick Overview for s100a4 抗体 (AA 1-200) (ABIN6940541)
抗原
See all s100a4 (S100A4) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1-200
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纯化方法
- Purified by Protein A/G
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免疫原
- Recombinant fragment (around aa 1-200) of human S100A4 protein (exact sequence is proprietary)
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亚型
- IgG1 kappa
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应用备注
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Positive Control: HeLa, A549 or A375 cells (IF/FACS). HeLa, A549, T98G or A375 cell lysate (WB). Human placenta tissue (IHC).
Known Application: Flow Cytometry (1-2 μg/million cells),Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))
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别名
- S100A4
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背景
- S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, 64 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.
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分子量
- 10-12kDa
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基因ID
- 6275
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UniProt
- P26447
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途径
- S100 Proteins
抗原
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