NFE2L1 抗体
Quick Overview for NFE2L1 抗体 (ABIN6940224)
抗原
See all NFE2L1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Purified by Protein A/G
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免疫原
- Recombinant full-length human NRF1 protein
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亚型
- IgG1 kappa
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anti-Nuclear Factor (erythroid-Derived 2)-Like 1 (NFE2L1) (AA 515-772) antibody
NFE2L1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor (erythroid-Derived 2)-Like 1 (NFE2L1) (AA 251-550) antibodyNFE2L1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor (erythroid-Derived 2)-Like 1 (NFE2L1) antibodyNFE2L1 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor (erythroid-Derived 2)-Like 1 (NFE2L1) (AA 677-771) antibodyNFE2L1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Nuclear Factor (erythroid-Derived 2)-Like 1 (NFE2L1) (N-Term) antibodyNFE2L1 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor (erythroid-Derived 2)-Like 1 (NFE2L1) (AA 575-624) antibodyNFE2L1 适用: 人, 小鼠, 大鼠, Cow, 犬, 兔, 猴, Hamster WB 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor (erythroid-Derived 2)-Like 1 (NFE2L1) (C-Term) antibodyNFE2L1 适用: 人, 小鼠, 大鼠, Cow, 兔, 豚鼠, 猴, Hamster, Bat WB 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor (erythroid-Derived 2)-Like 1 (NFE2L1) (C-Term) antibodyNFE2L1 适用: 人, 小鼠, 大鼠, Cow, 犬, 兔 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Positive Control: Ubiquitous expression, strongest in skeletal muscle.
Known Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- NFE2L1 (Nuclear Factor (erythroid-Derived 2)-Like 1 (NFE2L1))
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别名
- NFE2L1
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背景
- The NF-E2 DNA binding protein is composed of two subunits, p45 and MafK, and it regulates expression of globin genes in developing erythroid cells through interaction with Maf recognition elements (MAREs). A family of NF-E2 related proteins, which are collectively known as the Cap 'n' collar (CNC) family and include Nrf1 (also designated TCF11), Nrf2 and Nrf3, are bZIP transcription factors that heterodimerize with Maf proteins to bind MARE sequences. The Nrf proteins also bind the antioxidant response element (ARE) and are implicated in the regulation of detoxification enzymes and the oxidative stress response. They do so by heterodimerizing with Jun family members (c-Jun, JunB and JunD) to activate gene expression, specifically the detoxifying enzyme, NQO1. Nrf2 is widely expressed and is thought to translocate to the nucleus after treatment with xenobiotics and antioxidants, which stimulate its release from a repressor protein Keap1. Nrf3 is highly expressed in placenta, B cells and monocytes.
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分子量
- 30kDa (bZIP region), 65-120kDa (glycosylated)
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基因ID
- 4899
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UniProt
- Q16656
抗原
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