NPM1 抗体 (AA 185-287)
Quick Overview for NPM1 抗体 (AA 185-287) (ABIN6940219)
抗原
See all NPM1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
抗原表位
- AA 185-287
-
特异性
- Recognizes a 33 kDa glycoprotein, identified as Nucleophosmin (NPM). It is predominantly localized in the nucleus of cells in most tissues. NPM is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis. This phosphoprotein moves between the nucleus and the cytoplasm. It is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location. Mutations in this gene are associated with acute myeloid leukemia. The antibody may be a useful aid for classification of acute myeloid leukemia.
-
纯化方法
- Purified by Protein A/G
-
免疫原
- Recombinant human NPM1 protein fragment (aa185-287) (exact sequence is proprietary)
-
亚型
- IgG1 kappa
-
-
anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 1-294) antibody
NPM1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 3B2 unconjugated
anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 250-298), (C-Term) antibodyNPM1 适用: 人 WB, IF, IHC (p), IP, FACS 宿主: 兔 Polyclonal unconjugated
anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 20-154) antibodyNPM1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 212-292) antibodyNPM1 适用: 小鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (pThr199) antibodyNPM1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (C-Term) antibodyVerified NPM1 适用: 人, 小鼠 WB, IHC, ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 185-287) antibodyNPM1 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal rNPM1-1901 unconjugated Recombinant Antibody
anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 185-287) antibodyNPM1 适用: 人 WB, IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal NPM1-1902 unconjugated
anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 198-226) antibodyNPM1 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 7-33) antibodyNPM1 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Positive Control: K562 cells, Human colon or skin tissues (IHC).
Known Application: Immunofluorescence (1-2 μg/mL), ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined.
-
限制
- 仅限研究用
-
-
-
浓度
- 200 μg/mL
-
缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-80 °C
-
储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
-
有效期
- 24 months
-
-
- NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))
-
别名
- NPM1
-
分子量
- 33kDa
-
基因ID
- 4869
-
UniProt
- P06748
-
途径
- Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly
抗原
-
