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MYOD1 抗体 (AA 180-189)

This anti-MYOD1 antibody is a 小鼠 单克隆 antibody detecting MYOD1 in IF, FACS, IHC, StM 和 ISt. Suitable for 人, 小鼠, 大鼠 和 小鸡.
产品编号 ABIN6940159
发货至: 中国

Quick Overview for MYOD1 抗体 (AA 180-189) (ABIN6940159)

抗原

See all MYOD1 抗体
MYOD1 (Myogenic Differentiation 1 (MYOD1))

适用

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人, 小鼠, 大鼠, 小鸡

宿主

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小鼠

克隆类型

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单克隆

标记

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This MYOD1 antibody is un-conjugated

应用范围

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Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Staining Methods (StM), Immunostaining (ISt)

克隆位点

SPM427
  • 抗原表位

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    AA 180-189

    特异性

    Recognizes a phosphor-protein of 45 kDa, identified as MyoD1. The epitope of this MAb maps between amino acid 180-189 in the C-terminal of Mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm's tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing's sarcomas and alveolar soft part sarcomas.

    纯化方法

    Purified by Protein A/G

    免疫原

    Recombinant MyoD of Mouse origin with an epitope mapping to amino acids 180-189.

    亚型

    IgG1 kappa
  • 应用备注

    Positive Control: Rhabdomyosarcoma.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 1 mM EDTA Buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    限制

    仅限研究用
  • 浓度

    200 μg/mL

    缓冲液

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    有效期

    24 months
  • 抗原

    MYOD1 (Myogenic Differentiation 1 (MYOD1))

    别名

    MYOD1

    分子量

    45kDa

    基因ID

    4654

    UniProt

    P15172

    途径

    Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
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