Recombinant EpCAM 抗体 (Extracellular Domain)
Quick Overview for Recombinant EpCAM 抗体 (Extracellular Domain) (ABIN6939997)
抗原
See all EpCAM (EPCAM) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 100-224, Extracellular Domain
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特异性
- Epitope of this MAb is mapped between aa 202-212 of EGP40, which is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. Antibody to Ep-CAM has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
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免疫原
- Recombinant human EpCAM fragment from extracellular domain (around aa100-224) (exact sequence is proprietary)
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亚型
- IgG
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应用备注
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Positive Control: MCF-7 or HT29 cells. Breast tumor.
Known Application: Flow Cytometry (1-2 μg/million cells). Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
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别名
- TACSTD1
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分子量
- 40-43kDa
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基因ID
- 4072
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UniProt
- P16422
抗原
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