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EpCAM 抗体

This anti-EpCAM antibody is a 小鼠 单克隆 antibody detecting EpCAM in WB, FACS, IF, IHC 和 StM. Suitable for 人.
产品编号 ABIN6939993
发货至: 中国

Quick Overview for EpCAM 抗体 (ABIN6939993)

抗原

See all EpCAM (EPCAM) 抗体
EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

适用

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小鼠

克隆类型

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单克隆

标记

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This EpCAM antibody is un-conjugated

应用范围

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Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM)

克隆位点

PAN-EpCAM
  • 纯化方法

    Purified by Protein A/G

    免疫原

    Recombinant human EpCAM protein (full-length and fragments)

    亚型

    IgG kappa
  • 应用备注

    Positive Control: HT29 cells. Breast tumor.

    Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    限制

    仅限研究用
  • 浓度

    200 μg/mL

    缓冲液

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    有效期

    24 months
  • 抗原

    EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

    别名

    TACSTD1

    背景

    It is a cocktail of four highly specific monoclonal antibodies that recognize extracellular as well as intracellular domains of the epithelial cellular adhesion molecule (EpCAM). It is a 40-43 kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or EpCAM. EpCAM is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative. Epithelial antigen has also been suggested as a discriminator between basal cell and baso-squamous carcinomas, and squamous cell carcinoma of the skin.

    分子量

    40-43kDa

    基因ID

    4072

    UniProt

    P16422
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