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Recombinant EpCAM 抗体 (Extracellular Domain)

The 小鼠 单克隆 anti-EpCAM antibody (Clone rEGP40-1372) (ABIN6939985) specifically detects EpCAM in WB 和 IHC. The antibody is reactive with 人, 犬 和 Cat samples.
产品编号 ABIN6939985
发货至: 中国
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Quick Overview for Recombinant EpCAM 抗体 (Extracellular Domain) (ABIN6939985)

抗原

See all EpCAM (EPCAM) 抗体
EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

抗体类型

Recombinant Antibody

适用

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人, 犬, Cat

宿主

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小鼠

克隆类型

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  • 3
单克隆

标记

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This EpCAM antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC)

克隆位点

REGP40-1372
  • 抗原表位

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    AA 100-224, Extracellular Domain

    原理

    Recombinant Ep-CAM / CD326 (Extracellular Domain) (Epithelial Marker) Antibody

    特异性

    EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on basolateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

    纯化方法

    200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

    免疫原

    Recombinant fragment from the extracellular domain of human EpCAM protein (around aa100-224) (exact sequence is proprietary)

    亚型

    IgG1, kappa
  • 应用备注

    Positive Control: HT29 cells. Ovarian carcinoma.

    Known Application: Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    限制

    仅限研究用
  • 浓度

    200 μg/mL

    缓冲液

    Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    有效期

    24 months
  • 抗原

    EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

    别名

    TACSTD1

    背景

    Adenocarcinoma-associated Antigen, Cell Surface Glycoprotein Trop-1, EGP2, EGP314, EGP40, Epithelial Cell Adhesion Molecule, Epithelial Glycoprotein 314, ESA, KSA, TACD1, TROP1, Tumor-associated Calcium Signal Transducer 1 (TACSTD1), ECS-1, Epidermal Surface Antigen 1, ESA1, FLOT2, Flotillin-2, Membrane Component, Chromosome 17, Surface Marker-1 (M17S1), REG-1, Reggie-1, Reggie-2

    MCF-7 or HT29 cells. Human ovarian carcinoma. Human Intestine, HEK293.

    分子量

    40-43kDa

    基因ID

    4072, 542050

    UniProt

    P16422
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