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EpCAM 抗体 (Extracellular Domain)

This anti-EpCAM antibody is a 小鼠 单克隆 antibody detecting EpCAM in WB, FACS, IF, IHC 和 StM. Suitable for 人.
产品编号 ABIN6939978
发货至: 中国

Quick Overview for EpCAM 抗体 (Extracellular Domain) (ABIN6939978)

抗原

See all EpCAM (EPCAM) 抗体
EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

适用

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小鼠

克隆类型

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单克隆

标记

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This EpCAM antibody is un-conjugated

应用范围

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Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM)

克隆位点

EGP40-1373
  • 抗原表位

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    AA 77-202, Extracellular Domain

    纯化方法

    Purified by Protein A/G

    免疫原

    Recombinant human EpCAM protein fragment from extracellular domain (around aa77-202) (exact sequence is proprietary)

    亚型

    IgG2b kappa
  • 应用备注

    Positive Control: HT29 cells. Ovarian carcinoma.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Western Blot (0.5-1.0 μg/mL), Immunofluorescence (1-3 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    限制

    仅限研究用
  • 浓度

    200 μg/mL

    缓冲液

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    有效期

    24 months
  • 抗原

    EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

    别名

    TACSTD1

    背景

    EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

    分子量

    40-43kDa

    基因ID

    4072

    UniProt

    P16422
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