Recombinant Cytokeratin 19 抗体
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北京 101111
Quick Overview for Recombinant Cytokeratin 19 抗体 (ABIN6939940)
抗原
See all Cytokeratin 19 (KRT19) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Recombinant Cytokeratin 19 (KRT19) (Pancreatic Stem Cell Marker) Antibody
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特异性
- This MAb reacts with the rod domain of human cytokeratin-19 (CK19), a polypeptide of 40 kDa. Its epitope maps between amino acid 312-335. CK19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50 % -60 % of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.
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无交叉反应
- 雪貂, 大鼠
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纯化方法
- 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system.
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免疫原
- Recombinant human full-length KRT19 protein
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亚型
- IgG1, kappa
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anti-Keratin 19 (KRT19) antibody
KRT19 适用: 人 WB, ELISA, IHC (p), ICC, IP, ICFC 宿主: 小鼠 Monoclonal A53-B-A2 unconjugated
anti-Keratin 19 (KRT19) (AA 245-391) antibodyKRT19 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Keratin 19 (KRT19) (AA 1-403) antibodyKRT19 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Keratin 19 (KRT19) (AA 127-322) antibodyKRT19 适用: 人 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Keratin 19 (KRT19) (AA 245-391) antibodyKRT19 适用: 人 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal D1 unconjugated
anti-Keratin 19 (KRT19) (AA 241-400) antibodyKRT19 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Keratin 19 (KRT19) antibodyKRT19 适用: 人 WB, IHC (p), ICC, IP, ICFC 宿主: 小鼠 Monoclonal BA-17 unconjugated
anti-Keratin 19 (KRT19) (AA 80-400) antibodyKRT19 适用: 人, 大鼠 WB, IHC, FACS, ELISA 宿主: 小鼠 Monoclonal 2A1G9 unconjugated
anti-Keratin 19 (KRT19) (AA 274-400) antibodyKRT19 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 8H1A8 unconjugated
anti-Keratin 19 (KRT19) antibodyKRT19 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS 宿主: 小鼠 Monoclonal KRT19-800 unconjugated
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应用备注
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Positive Control: MCF-7, HeLa, Hep-G2 cells, Breast Cancer.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))
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别名
- KRT19
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背景
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k19, k1cs, Keratin 19 Keratin Type i 40kD, krt19
HeLa, Hep-G2 cells. Human breast cancer tissue., MCF-7. Human Skin.
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分子量
- 40kDa
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基因ID
- 3880, 654568
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UniProt
- P08727
抗原
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