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Recombinant Cytokeratin 19 抗体

This anti-Cytokeratin 19 antibody is a 小鼠 单克隆 antibody detecting Cytokeratin 19 in WB, IHC, IF, FACS 和 StM. Suitable for 人.
产品编号 ABIN6939940
发货至: 中国

Quick Overview for Recombinant Cytokeratin 19 抗体 (ABIN6939940)

抗原

See all Cytokeratin 19 (KRT19) 抗体
Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

抗体类型

Recombinant Antibody

适用

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小鼠

克隆类型

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单克隆

标记

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This Cytokeratin 19 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

克隆位点

RKRT19-799
  • 特异性

    This MAb reacts with the rod domain of human cytokeratin-19 (CK19), a polypeptide of 40 kDa. Its epitope maps between amino acid 312-335. CK19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50 % -60 % of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.

    无交叉反应

    雪貂, 大鼠

    纯化方法

    Purified by Protein A/G

    免疫原

    Recombinant human full-length KRT19 protein

    亚型

    IgG1 kappa
  • 应用备注

    Positive Control: MCF-7, HeLa, Hep-G2 cells, Breast Cancer.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    限制

    仅限研究用
  • 浓度

    200 μg/mL

    缓冲液

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    有效期

    24 months
  • 抗原

    Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

    别名

    KRT19

    分子量

    40kDa

    基因ID

    3880

    UniProt

    P08727
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