KRT8 抗体
Quick Overview for KRT8 抗体 (ABIN6939883)
抗原
See all KRT8 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Purified by Protein A/G
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免疫原
- Cytoskeleton preparation containing cytokeratin 8
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亚型
- IgG1 kappa
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anti-Keratin 8 (KRT8) antibody
KRT8 适用: 人, 小鼠 WB, IHC, FACS, ICC, IHC (fro) 宿主: 小鼠 Monoclonal M20 unconjugated
anti-Keratin 8 (KRT8) (AA 92-393) antibodyKRT8 适用: 人 WB, IHC, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated
anti-Keratin 8 (KRT8) (Ser726), (Ser731) antibodyKRT8 适用: 人 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Keratin 8 (KRT8) (pSer432) antibodyKRT8 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Keratin 8 (KRT8) (AA 91-381) antibodyKRT8 适用: 人, 大鼠, 猴, 犬 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 1B12 unconjugated
anti-Keratin 8 (KRT8) (AA 1-483) antibodyKRT8 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Keratin 8 (KRT8) antibodyKRT8 适用: 人, Cow, 兔, Pig, 绵羊 IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal B22-1 unconjugated
anti-Keratin 8 (KRT8) (AA 391-483) antibodyKRT8 适用: 人 WB, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 8A5D12 unconjugated
anti-Keratin 8 (KRT8) (Middle Region) antibodyKRT8 适用: 人, 大鼠, 小鼠, Cow, 兔, Pig, 犬, 豚鼠, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Keratin 8 (KRT8) (N-Term) antibodyKRT8 适用: 人, 大鼠, 小鼠, Cow, 犬, 豚鼠, 马, Saccharomyces cerevisiae WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Positive Control: MCF-7, HeLa, A431 cells. Skin, colon, lung or breast carcinoma.
Known Application: Western Blot (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- KRT8 (Keratin 8 (KRT8))
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别名
- KRT8
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背景
- Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Reportedly, anti-CK8 is useful for the differentiation of lobular ('ring-like, perinuclear') from ductal ('peripheral-predominant') carcinoma of the breast.
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分子量
- 52.5kDa
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基因ID
- 3856
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UniProt
- P05787
抗原
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