KRT6A/KRT6B/KRT6C (C-Term) 抗体
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- 抗原
- KRT6A/KRT6B/KRT6C
- 抗原表位
- C-Term
- 适用
- 人, 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Immunohistochemistry (IHC), Staining Methods (StM), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunostaining (ISt)
- 特异性
- This MAb recognizes a protein of 56 kDa, identified as cytokeratin 6 (CK6). In humans, multiple isoforms of Cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns, and Cytokeratin 6A is the dominant form in epithelial tissue. The gene encoding human Cytokeratin 6A maps to chromosome 12q13, and mutations in this gene are linked to several inheritable hair and skin pathologies. Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyper-proliferation-related keratins). Keratin 6 is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyper-proliferative situations. Epidermal injury results in activation of keratinocytes, which express CK6 and CK16. CK6 is strongly expressed in about 75 % of head and neck squamous cell carcinomas. Expression of CK6 is particularly associated with differentiation.
- 纯化方法
- Purified by Protein A/G
- 免疫原
- A synthetic peptide of 11 amino acids (GSSTIKYTTTS) from C-terminus of human keratin 6
- 克隆位点
- SPM269
- 亚型
- IgG2a kappa
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anti-KRT6A/KRT6B/KRT6C antibody
适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Positive Control: RAW, HeLa, Suprabasal cells of the outer hair follicle root sheath in skin. Basal cell carcinoma.
Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin/paraffin tissues is enhanced by digestion of tissue sections with pepsin (Sigma Cat. #P-7012) at 1 mg/mL Tris-HCl, pH 2.0 for 10-15 min at RT)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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