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- 抗原
- IGHA1 & IGHA2
- 适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Staining Methods (StM)
- 特异性
- This MAb is specific to heavy chain of IgA and shows minimal cross-reaction with heavy chains of other immunoglobulins. It is reactive with both IgA1 and IgA2 subclasses of Alpha heavy chain. It reacts with the third constant domain (CH3) of the alpha chain of IgA molecules. Immunoglobulins are four-chain, Y-shaped, monomeric structures comprised of two identical heavy chains and two identical light chains held together through inter-chain disulfide bonds. The chains form two domains, the Fab (antigen binding) fragment and the Fc (constant) fragment. Immunoglobulin A (IgA) is the main protein of the mucosal immune system. It is generated by B-cells in gut-associated lymphoid tissues. Daily production of IgA exceeds that of any of the other immunoglobulins.IgA exists mainly in dimers but can also exist as polymers or as monomers. Dimers and polymers contain a joining (J) chain that can be bound by the polymeric immunoglobulin receptor (pIgR) for transportation of the molecule to mucosal surfaces. The most common feature of plasmacytomas, and certain non-Hodgkin's lymphomas is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Purified human IgA
- 克隆位点
- HISA43
- 亚型
- IgG1 kappa
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anti-IGHA1 & IGHA2 antibody
适用: 人 IHC, StM 宿主: 小鼠 Monoclonal rHISA43 unconjugated Recombinant Antibody
anti-IGHA1 & IGHA2 (Internal Region) antibody适用: 人 IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-IGHA1 & IGHA2 antibody (CF®488A)适用: 人 IHC, FACS, IF, StM 宿主: 小鼠 Monoclonal IA761 CF®488A
anti-IGHA1 & IGHA2 antibody适用: 人 WB 宿主: 兔 Monoclonal unconjugated
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- 应用备注
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Positive Control: Daudi, 293T, Raji or hPBL cells. Tonsil or Spleen.
Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (0.5-1.0 μg/mL 30 min RT), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL 30 min RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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