ICAM-3/CD50 抗体
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- 抗原 See all ICAM-3/CD50 (ICAM3) 抗体
- ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This ICAM-3/CD50 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Staining Methods (StM)
- 特异性
- Recognizes an N-glycosylated glycoprotein of 120 kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (WS: IV V). Its epitope localizes in the D2 extracellular domain and is resistant to neuraminidase and proteases. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signalling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb inhibits primary mixed lymphocyte culture (MLC) but not secondary MLC, cytotoxicity or proliferation induced by mitogens. It blocks binding of NK1-L16 stimulated T cells to L cells expressing CD50. This MAb is excellent for staining of formalin/paraffin tissues.
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Stimulated human leukocytes
- 克隆位点
- 101-1D2
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product ICAM3 Primary Antibody
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anti-Intercellular Adhesion Molecule 3 (ICAM3) antibody
ICAM3 适用: 人 FACS, IHC (p), Func 宿主: 小鼠 Monoclonal MEM-04 unconjugated
anti-Intercellular Adhesion Molecule 3 (ICAM3) (AA 30-203) antibodyICAM3 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 2D11D1 unconjugated
anti-Intercellular Adhesion Molecule 3 (ICAM3) (AA 200-547) antibodyICAM3 适用: 人 WB, IF, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Intercellular Adhesion Molecule 3 (ICAM3) (AA 30-547) antibodyICAM3 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2F8 unconjugated
anti-Intercellular Adhesion Molecule 3 (ICAM3) (AA 46-197) antibodyICAM3 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Intercellular Adhesion Molecule 3 (ICAM3) (AA 30-130) antibodyICAM3 适用: 人 ELISA, IHC (p), ICC, IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Intercellular Adhesion Molecule 3 (ICAM3) (AA 259-491) antibodyICAM3 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Intercellular Adhesion Molecule 3 (ICAM3) antibodyICAM3 适用: 人 IF, IHC, StM, Bind 宿主: 小鼠 Monoclonal 186-2G9 unconjugated
anti-Intercellular Adhesion Molecule 3 (ICAM3) antibodyICAM3 适用: 人 IHC, StM, Bind 宿主: 兔 Monoclonal ICAM3-2873R unconjugated Recombinant Antibody
anti-Intercellular Adhesion Molecule 3 (ICAM3) (Internal Region) antibodyICAM3 适用: 人, 小鼠 WB, IF, IHC, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Positive Control: HL-60 or THP-1 cells. Lymph node and tonsil.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin/paraffin tissues Requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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