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ABO 抗体

This anti-ABO antibody is a 小鼠 单克隆 antibody detecting ABO in IHC, IF, AGG, H 和 StM. Suitable for 人.
产品编号 ABIN6939555
发货至: 中国

Quick Overview for ABO 抗体 (ABIN6939555)

抗原

See all ABO 抗体
ABO (Histo-blood group A transferase (ABO))

适用

  • 41
  • 13
  • 11
  • 1
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  • 1

宿主

  • 31
  • 10
小鼠

克隆类型

  • 29
  • 12
单克隆

标记

  • 25
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
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  • 1
This ABO antibody is un-conjugated

应用范围

  • 21
  • 17
  • 9
  • 7
  • 4
  • 4
  • 3
  • 2
  • 2
  • 1
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Immunohistochemistry (IHC), Immunofluorescence (IF), Agglutination (AGG), Haemagglutination (H), Staining Methods (StM)

克隆位点

HEB-20
  • 特异性

    The antibody HEB-20 reacts with human blood group B. The specificity of the antibody HEB-20 was confirmed by comparison of specificity and reactivity to standard reagent using 5.000 samples of blood. The MAb HEB-20 shows specific staining of erythrocytes and vascular epithelium of blood group B controls and no staining in group A controls. This MAb is applicable for tissue staining in tumor patients with blood groups B and AB. Blood group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.

    纯化方法

    Purified by Protein A/G

    免疫原

    Mixture of erythrocytes of group B and glyco protein fraction isolated from saliva of secretors with blood group B

    亚型

    IgG1 kappa
  • 应用备注

    Positive Control: KG1 cells. Human colorectal carcinoma tissues.

    Known Application: Agglutination, Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    限制

    仅限研究用
  • 浓度

    200 μg/mL

    缓冲液

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    有效期

    24 months
  • 抗原

    ABO (Histo-blood group A transferase (ABO))

    别名

    ABO

    基因ID

    28

    UniProt

    P16442
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