ABO 抗体
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- 抗原 See all ABO 抗体
- ABO (Histo-blood group A transferase (ABO))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This ABO antibody is un-conjugated
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应用范围
- ELISA, Immunofluorescence (IF), Agglutination (AGG), Haemagglutination (H), Coating (Coat)
- 特异性
- This antibody recognizes human blood group A (monofucosyl and difucosyl A antigens with chain types 1, 2, 3, 4, 5, 6) and Forssmann antigen. It is also reactive with the immuno-dominant A trisaccharide. Blood group antigen expression in human colon cancer was studied by means of two monoclonal antibodies of broad anti-A (HE-14) and anti-type 3 and type 4 chain-based A and H (HE-10) specificity. These antigens were proved to re-appear in tumors of the distal colon, the HE-10 antibody reacting more frequently (9 out of 12 samples) than HE-14 (5 out of 12 samples) and frequently with supra-nuclear staining of the cytoplasm probably in those places of the Golgi apparatus where carbohydrate antigens are synthesized. This staining pattern is characteristic of HE-10 in normal colonic mucosa as well. With HE-14, staining was often absent in less differentiated tumors, while HE-10 did react in such tumors. In some cases, these two antibodies gave different staining patterns in parallel sections from the same tissue sample, primarily at the cellular level. Three out of 12 cases showed blood group antigen expression in the mucosa of the distal colon adjacent to the tumor only when HE-10 Ab was used.
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Mixture of erythrocytes of blood group A1and glyco protein fraction isolated from the saliva of secretors with blood group A
- 克隆位点
- HE-14
- 亚型
- IgM kappa
- Top Product
- Discover our top product ABO Primary Antibody
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anti-Histo-blood group A transferase (ABO) (AA 54-354) antibody
ABO 适用: 人 IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Histo-blood group A transferase (ABO) (AA 273-354) antibodyABO 适用: 人 ELISA, WB 宿主: 小鼠 Monoclonal 1B7 unconjugated
anti-Histo-blood group A transferase (ABO) (N-Term) antibodyABO 适用: 人, 小鼠, 大鼠 ELISA, IHC, WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Histo-blood group A transferase (ABO) (AA 41-68), (N-Term) antibodyABO 适用: 人 WB 宿主: 小鼠 Monoclonal 593CT3-2-2 unconjugated
anti-Histo-blood group A transferase (ABO) antibodyABO 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Histo-blood group A transferase (ABO) antibodyABO 适用: 人 IHC, IF, AGG, H, StM 宿主: 小鼠 Monoclonal HEB-20 unconjugated
anti-Histo-blood group A transferase (ABO) antibodyABO 适用: 人, 小鼠, 大鼠 IHC, WB, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Histo-blood group A transferase (ABO) (AA 235-354) antibodyABO 适用: 人 ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-Histo-blood group A transferase (ABO) (AA 200-249) antibodyABO 适用: 人, 小鼠, 大鼠, 犬, 猴, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Histo-blood group A transferase (ABO) antibodyABO 适用: 人, 小鼠, 大鼠 IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Positive Control: KG1 cells. Human colorectal carcinoma tissues.
Known Application: ELISA (For coating, order Ab without BSA), Agglutination, Immunofluorescence (0.5-1 μg/mL), Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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- 抗原
- ABO (Histo-blood group A transferase (ABO))
- 别名
- ABO (ABO 产品)
- 别名
- A3GALNT antibody, A3GALT1 antibody, GTB antibody, NAGAT antibody, ABO, alpha 1-3-N-acetylgalactosaminyltransferase and alpha 1-3-galactosyltransferase antibody, ABO blood group (transferase A, alpha 1-3-N-acetylgalactosaminyltransferase, transferase B, alpha 1-3-galactosyltransferase) antibody, ABO antibody, Abo antibody
- 基因ID
- 28
- UniProt
- P16442
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