GFAP 抗体 (AA 101-200)
Quick Overview for GFAP 抗体 (AA 101-200) (ABIN6939507)
抗原
See all GFAP 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 101-200
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特异性
- This MAb recognizes a protein of ~50 kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
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纯化方法
- Purified by Protein A/G
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免疫原
- Recombinant human GFAP protein fragment (around aa 101-200) (exact sequence is proprietary)
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亚型
- IgG1 kappa
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应用备注
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Positive Control: T98G cells (FACS). Brain lysate (WB). Brain or Astrocytoma (IHC).
Known Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- GFAP (Glial Fibrillary Acidic Protein (GFAP))
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别名
- GFAP
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分子量
- ~50kDa
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基因ID
- 2670
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UniProt
- P14136
抗原
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