GAD 抗体 (AA 72-135)
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北京 101111
Quick Overview for GAD 抗体 (AA 72-135) (ABIN6939482)
抗原
See all GAD (GAD1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 72-135
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特异性
- This MAb recognizes a protein of 67 kDa, which is identified as glutamic acid decarboxylase 1 (GDA1). There are two forms of glutamic acid decarboxylases (GADs) that are found in the brain: GAD65 (also known as GAD2) and GAD67 (also known as GAD1. GAD65 and GAD67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate-limiting step in the production of GABA (-aminobutyric acid) from L-glutamic acid. Although both GAD's are found in the brain, GAD65 localizes to synaptic vesicle membranes in nerve terminals, while GAD67 is distributed throughout the cell. GAD67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD65 will transiently activate to assist in GABA production. The loss of GAD65 is detrimental and can impair GABA neurotransmission, however the loss of GAD67 is lethal.
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纯化方法
- Purified by Protein A/G
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免疫原
- Recombinant human GAD1 (GAD67) protein fragment (around aa 72-135) (exact sequence is proprietary)
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亚型
- IgG2b kappa
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anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (N-Term) antibody
GAD1 适用: 人 WB, IHC, ICC, IHC (p), IF, IHC (fro), IHC (ff), IHC (wm) 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (AA 1-97) antibodyGAD1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (AA 455-594) antibodyGAD1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (Internal Region) antibodyVerified GAD1 适用: 人 WB, ELISA, FACS, IF 宿主: 山羊 Polyclonal unconjugated
anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (C-Term) antibodyGAD1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (AA 72-135) antibodyGAD1 适用: 人 WB, IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal GAD1-2391 unconjugated
anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (AA 14-122) antibodyGAD1 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (C-Term) antibodyGAD1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (AA 1-200) antibodyGAD1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Positive Control: T98G or K-562 or HEK293 cells. Pancreas.
Known Application: ELISA (For coating, order antibody without BSA), Flow Cytometry (1-2 μg/million cells), Western Blot (0.5-1 μg/mL), Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))
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别名
- GAD1
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分子量
- 67kDa
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基因ID
- 2571
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UniProt
- Q99259
抗原
-
