alpha Fetoprotein 抗体
Quick Overview for alpha Fetoprotein 抗体 (ABIN6939240)
抗原
See all alpha Fetoprotein (AFP) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
特异性
- It recognizes an oncofetal glycoprotein with a single chain of 70 kDa, which is identified as alpha fetoprotein (AFP) (ISOBM TD-2 workshop). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.
-
无交叉反应
- 牛, 犬, 大鼠
-
纯化方法
- Purified by Protein A/G
-
免疫原
- Alpha feto protein (AFP) Purified from serum of a hepatoma patient
-
亚型
- IgG1 kappa
-
-
-
-
应用备注
-
Positive Control: Hep-G2 cells. Fetal liver or hepatocellular carcinoma.
Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
-
限制
- 仅限研究用
-
-
-
浓度
- 200 μg/mL
-
缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-80 °C
-
储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
-
有效期
- 24 months
-
-
- alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))
-
别名
- AFP
-
分子量
- 70kDa
-
基因ID
- 174
-
UniProt
- P02771
-
途径
- C21-Steroid Hormone Metabolic Process
抗原
-