alpha Fetoprotein 抗体
Quick Overview for alpha Fetoprotein 抗体 (ABIN6939239)
抗原
See all alpha Fetoprotein (AFP) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- It recognizes an oncofetal glycoprotein with a single chain of 70 kDa, which is identified as alpha fetoprotein (AFP). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. The yolk sac and the liver produce AFP during fetal life. AFP expression in adults is often associated with hepatoma or teratoma. However, hereditary persistence of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the AFP and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. AFP is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of AFP in amniotic fluid is used to measure renal loss of protein to screen for spinal bifida and anencephaly.
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纯化方法
- Purified by Protein A/G
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免疫原
- Human Alpha feto protein
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亚型
- IgG1 kappa
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anti-alpha-Fetoprotein (AFP) (AA 500-609) antibody
AFP 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 1G7 unconjugated
anti-alpha-Fetoprotein (AFP) (AA 399-605) antibodyAFP 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-alpha-Fetoprotein (AFP) antibodyAFP 适用: 人, 犬, 猴 WB, IHC, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal 2A9 unconjugated
anti-alpha-Fetoprotein (AFP) (AA 360-609) antibodyAFP 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-alpha-Fetoprotein (AFP) (AA 31-171) antibodyAFP 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-alpha-Fetoprotein (AFP) (AA 409-611) antibodyAFP 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-alpha-Fetoprotein (AFP) antibodyAFP 适用: 人, 大鼠 WB, ELISA, IHC (p), IF (p) 宿主: 小鼠 Monoclonal A2 unconjugated
anti-alpha-Fetoprotein (AFP) (C-Term) antibodyAFP 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-alpha-Fetoprotein (AFP) (AA 19-397) antibodyAFP 适用: 人 WB, IHC, IF 宿主: 小鼠 Monoclonal OTI2F11 unconjugated
anti-alpha-Fetoprotein (AFP) (AA 31-171) antibodyAFP 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C1 unconjugated
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应用备注
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Positive Control: Hep-G2 cells. Fetal liver or hepatocellular carcinoma.
Known Application: Flow Cytometry (0.5-1 μg/million cells in 0.1ml), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))
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别名
- AFP
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分子量
- 70kDa
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基因ID
- 174
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UniProt
- P02771
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途径
- C21-Steroid Hormone Metabolic Process
抗原
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