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Recombinant CNN1 抗体

This anti-CNN1 antibody is a 小鼠 单克隆 antibody detecting CNN1 in FACS, IHC, WB 和 StM. Suitable for 人 和 大鼠.
产品编号 ABIN6939165
发货至: 中国

Quick Overview for Recombinant CNN1 抗体 (ABIN6939165)

抗原

See all CNN1 抗体
CNN1 (Calponin 1 (CNN1))

抗体类型

Recombinant Antibody

适用

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人, 大鼠

宿主

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小鼠

克隆类型

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单克隆

标记

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This CNN1 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), Staining Methods (StM)

克隆位点

RCNN1-832
  • 特异性

    Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist, a 34 kDa form and a 29 kDa form. Expression of the 29 kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this MAb reacts with only the 34 kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.

    纯化方法

    Purified by Protein A/G

    免疫原

    Recombinant full-length human CNN1 protein

    亚型

    IgG1 kappa
  • 应用备注

    Positive Control: K562 cells, Myoepithelial cells in breast ducts.

    Known Application: Western Blot (0.5-1 μg/mL), Flow Cytometry (1-2 μg/million cells), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    限制

    仅限研究用
  • 浓度

    200 μg/mL

    缓冲液

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    有效期

    24 months
  • 抗原

    CNN1 (Calponin 1 (CNN1))

    别名

    CNN1

    分子量

    34kDa

    基因ID

    1264

    UniProt

    P51911
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