Recombinant CNN1 抗体
Quick Overview for Recombinant CNN1 抗体 (ABIN6939165)
抗原
See all CNN1 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist, a 34 kDa form and a 29 kDa form. Expression of the 29 kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this MAb reacts with only the 34 kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.
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纯化方法
- Purified by Protein A/G
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免疫原
- Recombinant full-length human CNN1 protein
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亚型
- IgG1 kappa
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应用备注
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Positive Control: K562 cells, Myoepithelial cells in breast ducts.
Known Application: Western Blot (0.5-1 μg/mL), Flow Cytometry (1-2 μg/million cells), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- CNN1 (Calponin 1 (CNN1))
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别名
- CNN1
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分子量
- 34kDa
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基因ID
- 1264
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UniProt
- P51911
抗原
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