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CKB 抗体

The 小鼠 单克隆 anti-CKB antibody is suitable to detect CKB in samples from 人, 大鼠, 小鼠, 豚鼠 和 Hamster. It has been validated for WB 和 IHC.
产品编号 ABIN6939145
发货至: 中国
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for CKB 抗体 (ABIN6939145)

抗原

See all CKB 抗体
CKB (Creatine Kinase, Brain (CKB))

适用

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人, 大鼠, 小鼠, 豚鼠, Hamster

宿主

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小鼠

克隆类型

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单克隆

标记

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This CKB antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC)

克隆位点

CPTC-CKB-2
  • 原理

    Creatine Phosphokinase-BB (CK-BB) Antibody

    特异性

    Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB. This MAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB.

    纯化方法

    200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

    免疫原

    Recombinant human full-length CKB protein

    亚型

    IgG2b, kappa
  • 应用备注

    Positive Control: SH-SY5Y, HEK-293 and Cerebellum.

    Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    限制

    仅限研究用
  • 浓度

    200 μg/mL

    缓冲液

    Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    有效期

    24 months
  • 抗原

    CKB (Creatine Kinase, Brain (CKB))

    别名

    CKB

    背景

    BCK, Brain creatine kinase, Ckb, Creatine kinase B chain, Creatine kinase B-type, Creatine Kinase BB Isoenzyme, Creatine phosphokinase BB, Epididymis luminal protein 211, Epididymis secretory protein Li 29, HEL 211

    Y79 or HEK-293T cells. Human cerebellum. Human Brain, Mouse Brain, Rat Brain, Hamster Brain, Guinea pig Brain, Human Stomach, Mouse Stomach, Rat Stomach, Hamster Stomach or Guinea pig Stomach tissue

    分子量

    42kDa

    基因ID

    1152, 173724

    UniProt

    P12277
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