WT1 抗体 (AA 1-181)
Quick Overview for WT1 抗体 (AA 1-181) (ABIN6938782)
抗原
See all WT1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1-181
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特异性
- Recognizes a 47-55kDa-tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilm's tumors.WT1, a sporadic and familial pediatric kidney tumor, is genetically heterogeneous. Wilm's tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilm's tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.
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交叉反应 （详细）
- Human. Rat. Weakly reacts with Mouse.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant protein corresponding to residues 1-181 of human WT1.
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亚型
- IgG1 kappa
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anti-Wilms Tumor 1 (WT1) (AA 1-302) antibody
WT1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Wilms Tumor 1 (WT1) antibodyWT1 适用: 人 IHC, WB 宿主: 兔 Monoclonal unconjugated
anti-Wilms Tumor 1 (WT1) (AA 1-181) antibodyWT1 适用: 人, 猴 IHC, FACS, WB, ELISA, ICC 宿主: 小鼠 Monoclonal 7F9E8 unconjugated
anti-Wilms Tumor 1 (WT1) antibodyWT1 适用: 人, 大鼠, 小鼠 IHC, StM 宿主: 小鼠 Monoclonal rWT1-857 unconjugated Recombinant Antibody
anti-Wilms Tumor 1 (WT1) (AA 1-181) antibodyWT1 适用: 人, 猴 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 7F9G7H2 unconjugated
anti-Wilms Tumor 1 (WT1) (C-Term) antibodyWT1 适用: 人, 大鼠, 小鼠, 兔, 犬, 猴, Bat, 小鸡, 非洲爪蟾 IHC, WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Wilms Tumor 1 (WT1) (AA 1-181) antibodyWT1 适用: 人, 大鼠, 小鼠 IHC, StM 宿主: 小鼠 Monoclonal 6F-H2 unconjugated
anti-Wilms Tumor 1 (WT1) (AA 1-302) antibodyWT1 适用: 人 IF, WB 宿主: 兔 Polyclonal unconjugated
anti-Wilms Tumor 1 (WT1) (AA 314-479) antibodyWT1 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 5G11A5 unconjugated
anti-Wilms Tumor 1 (WT1) (AA 346-375) antibodyWT1 适用: 人, 小鼠 IF, FACS, WB, IHC (p) 宿主: 兔 Polyclonal RB18841 unconjugated
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应用备注
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Known_Application: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
Positive_Control: K562 cells. Kidney, Testis, Wilm's Tumor or Mesothelioma.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- WT1 (Wilms Tumor 1 (WT1))
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别名
- WT1
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背景
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WT1; AWT1; FWT1; GUD; NPHS4; WAGR; Wilms tumor 1,Wilm's Tumor 1 (WT1) (Wilm's Tumor & Mesothelial Marker)
Cellular localisation: Nuclear and Cytoplasmic -
分子量
- 47-55kDa
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基因ID
- 7490, 591980
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UniProt
- P19544
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途径
- Tube Formation
抗原
-
