VTCN1 抗体
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- 抗原 See all VTCN1 抗体
- VTCN1 (V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This VTCN1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 特异性
- T cell activation and immune function are regulated by the innate immune system through positive and negative costimulatory proteins. One such protein, B7-H4 (B7-homolog 4), belongs to the B7 immunoglobulin superfamily of ligand-lymphocyte interacting proteins. Expressed primarily on the membrane of lymphoid cells, B7-H4 is an immuno-inhibitory protein that interacts with receptors on the surface of T lymphocytes, thus mediating cellular and humoral immune responses. Overexpression of the B7-H4 protein is associated with certain malignancies, including ovarian and breast cancer, as its interaction with T cells suppresses tumor-associated immunity. Current research suggests that, similar to Mucin 16 (CA-125), B7-H4 may be a useful biomarker for the early detection of ovarian cancer.
- 交叉反应 (详细)
- Human.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- A recombinant fragment of human B7-H4 protein (exact sequence is proprietary)
- 克隆位点
- B7H4-1788
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product VTCN1 Primary Antibody
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- 应用备注
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Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Flow Cytometry (1-2 μg/million cells in 0.1ml), ,Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: HeLa, SKBr-3 or MCF-7 cells. Human ovary, pancreas, placenta or spleen.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
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- 抗原
- VTCN1 (V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1))
- 别名
- VTCN1 (VTCN1 产品)
- 别名
- B7h4 antibody, B7s1 antibody, B7x antibody, BC032925 antibody, B7-H4 antibody, B7H4 antibody, B7S1 antibody, B7X antibody, B7h.5 antibody, PRO1291 antibody, VCTN1 antibody, RGD1311204 antibody, V set domain containing T cell activation inhibitor 1 L homeolog antibody, V-set domain containing T cell activation inhibitor 1 antibody, V-set domain containing T-cell activation inhibitor 1 antibody, V set domain containing T cell activation inhibitor 1 antibody, vtcn1.L antibody, VTCN1 antibody, vtcn1 antibody, Vtcn1 antibody
- 背景
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B7 family member, H4, B7 homolog 4, B7 superfamily member 1, B7-H4, B7S1, Immune costimulatory protein B7-H4, T-cell costimulatory molecule B7x, V-set domain-containing T-cell activation inhibitor 1, VCTN1,B7-H4 (Immuno-Inhibitory Protein)
Cellular localisation: Cell Surface and Cytoplasmic - 分子量
- 35kDa
- 基因ID
- 79679, 546434
- UniProt
- Q7Z7D3
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