Recombinant UCHL1 抗体
Quick Overview for Recombinant UCHL1 抗体 (ABIN6938724)
抗原
See all UCHL1 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This MAb reacts with a protein of 20-30kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinson's disease.
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交叉反应 (详细)
- Human. Dog. Rat. Shows a broad species reactivity.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant full-length human UCHL1 protein
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亚型
- IgG1 kappa
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应用备注
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Known_Application: Western Blot (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: Cerebellum.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))
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别名
- UCHL1
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背景
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Gracile Axonal Dystrophy, Neuron Cytoplasmic Protein 9.5, Park5, Parkinson Disease 5, PGP95, Protein Gene Product 9.5, Ubiquitin Carboxyl-terminal Esterase L1, Ubiquitin Carboxyl-terminal Hydrolase Isozyme L1, Ubiquitin Thioesterase L1, Ubiquitin Thiolesterase L1,PGP9.5 / UchL1 (pan-Neuronal Marker)
Cellular localisation: Cytoplasmic. Endoplasmic Reticulum membrane -
分子量
- 20-30kDa
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基因ID
- 7345, 518731
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UniProt
- P09936
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途径
- Feeding Behaviour
抗原
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