Transgelin 抗体
Quick Overview for Transgelin 抗体 (ABIN6938512)
抗原
See all Transgelin (TAGLN) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This MAb recognizes a 22 kDa protein, identified as Transgelin, also designated SM22-alpha. It may cross-react with SM22-beta. Transgelin is expressed abundantly in smooth muscle cells. The human transgelin gene encodes a 201 amino acid protein that contains nuclear factor-binding motifs known to regulate transcription in smooth muscle. During embryogenesis, transgelin is expressed in smooth, cardiac and skeletal muscle, but is restricted during late fetal development and adulthood to all vascular and visceral smooth muscle cells and low levels of expression in heart. Transgelin is down regulated in several transformed cell lines, indicating that a reduction of transgelin expression may be an early indicator of the onset of transformation. Transgelin also binds Actin, causing Actin fibers to gel within minutes of binding. Binding of transgelin to Actin occurs at a ratio of 1:6 Actin monomers.
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交叉反应 （详细）
- Human. Cow. Pig. Rabbit. Mouse.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant full-length human transgelin (TAGLN) protein.
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亚型
- IgG1 kappa
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anti-Transgelin (TAGLN) (C-Term) antibody
Verified TAGLN 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Transgelin (TAGLN) (AA 1-201) antibodyTAGLN 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Transgelin (TAGLN) antibodyTAGLN 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Transgelin (TAGLN) antibodyKO Validated TAGLN 适用: 人 WB, IF, IHC (p), IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Transgelin (TAGLN) (AA 1-201) antibodyTAGLN 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1E2 unconjugated
anti-Transgelin (TAGLN) (AA 2-201) antibodyTAGLN 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Transgelin (TAGLN) (AA 19-112) antibodyTAGLN 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1C4 unconjugated
anti-Transgelin (TAGLN) (AA 1-201) antibodyTAGLN 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3A3 unconjugated
anti-Transgelin (TAGLN) (AA 24-51), (N-Term) antibodyTAGLN 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB19881 unconjugated
anti-Transgelin (TAGLN) (C-Term) antibodyTAGLN 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Positive_Control: U-2 OS, Hs68 or WI 38 cells. Colon carcinoma.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- Transgelin (TAGLN)
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别名
- TAGLN
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背景
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2 kDa actin-binding protein, Human 22 kDa smooth muscle protein, Protein WS3-10, SMCC, Smooth muscle protein 22-alpha (SM22-alpha), TAGLN, TAGLN1, transgelin, Transgelin variant 2, WS3-10,Transgelin (SM22-alpha)
Cellular localisation: Cytoplasmic -
分子量
- 22kDa
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基因ID
- 6876, 410977
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UniProt
- Q01995
抗原
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