Recombinant Spectrin, Beta, Non-erythrocytic 2 (SPTBN2) (AA 356-475) 抗体
Quick Overview for Recombinant Spectrin, Beta, Non-erythrocytic 2 (SPTBN2) (AA 356-475) 抗体 (ABIN6938448)
抗原
See all Spectrin, Beta, Non-erythrocytic 2 (SPTBN2) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 356-475
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特异性
- Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II) four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta. Spectrin beta 3 is primarily expressed in nervous tissues with highest expression levels in the cerebellum, where it is found in Purkinje cell soma and dendrites.
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交叉反应 (详细)
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant human SPTBN2 fragment (aa356-475) (exact sequence is proprietary)
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亚型
- IgG
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应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), (Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HeLa cells. Pancreas or Liver.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- Spectrin, Beta, Non-erythrocytic 2 (SPTBN2)
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别名
- SPTBN2
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背景
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Beta III spectrin, SCA5, Spectrin beta chain brain 2, Spectrin beta non-erythrocytic 2, Spectrin non-erythroid beta chain 2, Spinocerebellar ataxia 5, SPTBN2,Spectrin beta III (SPTBN2)
Cellular localisation: Cell Surface -
分子量
- 246kDa
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基因ID
- 6712, 26915
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UniProt
- O15020
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途径
- Regulation of Actin Filament Polymerization, Synaptic Vesicle Exocytosis
抗原
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