Spectrin beta Chain, Erythrocyte (SPTB) 抗体
Quick Overview for Spectrin beta Chain, Erythrocyte (SPTB) 抗体 (ABIN6938442)
抗原
See all Spectrin beta Chain, Erythrocyte (SPTB) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Spectrin is a cytoskeletal protein that lines the intracellular side of the plasma membrane of many cell types in pentagonal or hexagonal arrangements, forming a scaffolding and playing an important role in maintenance of plasma membrane integrity and cytoskeletal structure. In certain types of brain injury such as diffuse axonal injury, spectrin is irreversibly cleaved by the proteolytic enzyme calpain, destroying the cytosketelon. Spectrin cleavage causes the membrane to form blebs and ultimately to be degraded, usually leading to cell death.
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交叉反应 （详细）
- Human. Mouse. Rat.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Red cell membranes
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亚型
- IgG2a kappa
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anti-Spectrin beta Chain, Erythrocyte (SPTB) antibody
SPTB 适用: 人, 小鼠, 大鼠 WB, IF, IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal RG-26 unconjugated
anti-Spectrin beta Chain, Erythrocyte (SPTB) antibodySPTB 适用: 人 WB, IP, IHC 宿主: 小鼠 Monoclonal DB2 unconjugated
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应用备注
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Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Western Blot (1-2 μg/mL), ,Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) ,(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: Jurkat whole cell lysate (WB). Human colon carcinoma tissue (IHC).
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- Spectrin beta Chain, Erythrocyte (SPTB)
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别名
- SPTB
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背景
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Beta-I spectrin, EL3, erythrocyte, HS2, SPTB1, HSPTB1, membrane cytoskeletal protein, spectrin beta (erythrocytic),Spectrin beta I (SPTB)
Cellular localisation: Cell Surface -
分子量
- 246kDa
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基因ID
- 6710, 26915
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UniProt
- O15020
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途径
- Regulation of Actin Filament Polymerization
抗原
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